2c1x

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File:2c1x.gif


2c1x, resolution 1.90Å

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STRUCTURE AND ACTIVITY OF A FLAVONOID 3-O GLUCOSYLTRANSFERASE REVEALS THE BASIS FOR PLANT NATURAL PRODUCT MODIFICATION

OverviewOverview

Glycosylation is a key mechanism for orchestrating the bioactivity, metabolism and location of small molecules in living cells. In plants, a, large multigene family of glycosyltransferases is involved in these, processes, conjugating hormones, secondary metabolites, biotic and abiotic, environmental toxins, to impact directly on cellular homeostasis. The red, grape enzyme UDP-glucose:flavonoid 3-O-glycosyltransferase (VvGT1) is, responsible for the formation of anthocyanins, the health-promoting, compounds which, in planta, function as colourants determining flower and, fruit colour and are precursors for the formation of pigmented polymers in, red wine. We show that VvGT1 is active, in vitro, on a range of, flavonoids. VvGT1 is somewhat promiscuous with respect to donor sugar, ... [(full description)]

About this StructureAbout this Structure

2C1X is a [Single protein] structure of sequence from [Vitis vinifera] with UDP and B3P as [ligands]. Active as [Flavonol 3-O-glucosyltransferase], with EC number [2.4.1.91]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Structure of a flavonoid glucosyltransferase reveals the basis for plant natural product modification., Offen W, Martinez-Fleites C, Yang M, Kiat-Lim E, Davis BG, Tarling CA, Ford CM, Bowles DJ, Davies GJ, EMBO J. 2006 Mar 22;25(6):1396-405. Epub 2006 Feb 16. PMID:16482224

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