1afi

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1afi

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STRUCTURE OF THE REDUCED FORM OF MERP, THE PERIPLASMIC PROTEIN FROM THE BACTERIAL MERCURY DETOXIFICATION SYSTEM, NMR, 20 STRUCTURES

OverviewOverview

Bacteria carrying plasmids with the mer operon, which encodes the proteins, responsible for the bacterial mercury detoxification system, have the, ability to transport Hg(II) across the cell membrane into the cytoplasm, where it is reduced to Hg(0). This is significant because metallic mercury, is relatively nontoxic and volatile and thus can be passively eliminated., The structures of the reduced and mercury-bound forms of merP, the, periplasmic protein, which binds Hg(II) and transfers it to the membrane, transport protein merT, have been determined in aqueous solution by, multidimensional NMR spectroscopy. The 72-residue merP protein has a, betaalpha betabeta alphabeta fold with the two alpha helices overlaying a, four-strand antiparallel beta sheet. Structural differences between the, ... [(full description)]

About this StructureAbout this Structure

1AFI is a [Single protein] structure of sequence from [Shigella flexneri]. Structure known Active Site: HMA. Full crystallographic information is available from [OCA].

ReferenceReference

Structures of the reduced and mercury-bound forms of MerP, the periplasmic protein from the bacterial mercury detoxification system., Steele RA, Opella SJ, Biochemistry. 1997 Jun 10;36(23):6885-95. PMID:9188683

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