CRYSTAL STRUCTURE OF H129V MUTANT OF ALCALIGENES XYLOSOXIDANS NITRITE REDUCTASE

File:1wae.gif


1wae, resolution 1.95Å

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OverviewOverview

Copper nitrite reductases contain both an electron-transfer type 1 Cu site, and a catalytic type 2 Cu site. We have mutated one of the type 2 copper, ligating histidines to observe the effect on catalytic turnover. This, mutation has created a unique site where Cu is ligated by 2 His Nepsilon2, atoms alone.

About this StructureAbout this Structure

1WAE is a [Single protein] structure of sequence from [Achromobacter xylosoxidans] with CU and ZN as [ligands]. Active as [Transferred entry: 1.7.2.1], with EC number [1.7.99.3]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Observation of an unprecedented Cu Bis-His site: crystal structure of the H129V mutant of nitrite reductase., Ellis MJ, Antonyuk SV, Strange RW, Sawers G, Eady RR, Hasnain SS, Inorg Chem. 2004 Nov 29;43(24):7591-3. PMID:15554622

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