8ruc
ACTIVATED SPINACH RUBISCO COMPLEXED WITH 2-CARBOXYARABINITOL BISPHOSPHATE
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OverviewOverview
Ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) from spinach is, a hexadecamer (L8S8, Mr = 550,000) consisting of eight large (L, 475, residues) and eight small subunits (S, 123 residues). High-resolution data, collection on crystals with large unit cells is not a trivial task due to, the effect of radiation damage and the large number of overlapping, reflections when conventional data collection methods are used. In order, to minimise these effects, data on rubisco were collected with a giant, Weissenberg camera at long crystal to image-plate distances at the, synchrotron of the Photon Factory, Japan. Relative to conventional data, sets, this experimental arrangement allowed a 20 to 30-fold reduction of, the X-ray dose/exposure time for data collection. This paper describes ... [(full description)]
About this StructureAbout this Structure
8RUC is a [Protein complex] structure of sequences from [Spinacia oleracea] with MG and CAP as [ligands]. This structure superseeds the now removed PDB entry 8RUB. The following page contains interesting information on the relation of 8RUC with [Rubisco]. Active as [Ribulose-bisphosphate carboxylase], with EC number [4.1.1.39]. Structure known Active Sites: ACA, ACC, ACE and ACG. Full crystallographic information is available from [OCA].
ReferenceReference
Large structures at high resolution: the 1.6 A crystal structure of spinach ribulose-1,5-bisphosphate carboxylase/oxygenase complexed with 2-carboxyarabinitol bisphosphate., Andersson I, J Mol Biol. 1996 May 31;259(1):160-74. PMID:8648644
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