1bp7
GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA
|
OverviewOverview
The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.
About this StructureAbout this Structure
1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068
Page seeded by OCA on Thu Feb 21 11:57:37 2008