159d
SIDE BY SIDE BINDING OF TWO DISTAMYCIN A DRUGS IN THE MINOR GROOVE OF AN ALTERNATING B-DNA DUPLEX
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OverviewOverview
Here we report the 1.8A X-ray structure of a 2:1 drug-DNA complex between distamycin A and an alternating B-DNA octamer duplex d(ICICICIC)2. The two distamycin A molecules are bound side by side with dyad symmetry in an antiparallel orientation in the expanded minor groove. The amides of each drug molecule are hydrogen bonded to the minor groove base atoms of only one DNA strand. The complex not only shows binding of two drug molecules, but the DNA duplex also exhibits striking low-high alternations in the helical twist angles, the sugar puckering and the phosphate conformations, providing the basis for a new model for an alternating B-DNA with a dinucleotide repeat.
About this StructureAbout this Structure
159D is a Protein complex structure of sequences from [1] with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Binding of two distamycin A molecules in the minor groove of an alternating B-DNA duplex., Chen X, Ramakrishnan B, Rao ST, Sundaralingam M, Nat Struct Biol. 1994 Mar;1(3):169-75. PMID:7656035
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