2bsk

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File:2bsk.jpg


2bsk, resolution 3.3Å

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CRYSTAL STRUCTURE OF THE TIM9 TIM10 HEXAMERIC COMPLEX

OverviewOverview

Import of proteins into mitochondria occurs by coordinated actions of, preprotein translocases in the outer and inner membranes. Tim9 and Tim10, are translocase components of the intermembrane space, related to, deafness-dystonia peptide 1 (DDP1). They coassemble into a hexamer, TIM9.10, which captures and chaperones precursors of inner membrane, metabolite carriers as they exit the TOM channel in the outer membrane., The crystal structure of TIM9.10 reveals a previously undescribed, alpha-propeller topology in which helical "blades" radiate from a narrow, central pore lined with polar residues. The propeller blades are, reminiscent of "tentacles" in chaperones Skp and prefoldin. In each, TIM9.10 subunit, a signature "twin CX3C" motif forms two intramolecular, disulfides. There is no obvious binding pocket for precursors, which we, suggest employ the chaperone-like tentacles of TIM9.10 as surrogate lipid, contacts. The first reported crystal structure of a mitochondrial, translocase assembly provides insights into selectivity and regulation of, precursor import.

About this StructureAbout this Structure

2BSK is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller., Webb CT, Gorman MA, Lazarou M, Ryan MT, Gulbis JM, Mol Cell. 2006 Jan 6;21(1):123-33. PMID:16387659

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