CAP-Gly domain
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| 1tov, resolution 1.77Å () | |||||||||
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| Gene: | F53F4.3 (Caenorhabditis elegans) | ||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum, TOPSAN | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
CAP-Gly domains in cytoskeletal proteinsCAP-Gly domains in cytoskeletal proteins
ActivityActivity
The cytoskeletal network contains Cytoskeleton-Associated Proteins (CAPs), including CLIP-170 and dynactins, that function in the organization of microtubules and in the transportation of vesicles and organelles. CAPs generally contain glycine-rich domains with a well-conserved GKNDG sequence motif presumably involved in protein-protein binding.
StructureStructure
The first crystal structure of a CAP-Gly domain (PDP file 1lpl), from Caenorhabditis elegans protein F53F4.3, revealed a novel protein fold containing a small antiparallel beta-barrel, tightly curved on one side and with a gap on the other side. The GKNDG sequence is in two consecutive turns of a surface loop, forming the entrance to a groove. Residues in the groove are highly conserved among the related sequences[1]. In the crystal, a dimer is formed by binding of the C-terminus of each chain into the GKNDG groove of the other chain.
1TOV is a rebuilt and re-refined version of the same dataset from 1LPL, giving an improvement of about 4% in Rfree, identifying a sulfate (seen in the interactive image), and adding an additional turn of ordered helix toward the N-terminal end of the domain fragment. Both are from the SouthEast Collaboratory for Structural Genomics (SECSG), solved by single wavelength sulfur-anomalous phasing. The new 1tov structure was produced as part of a systematic 30-strucutre test of the then new MolProbity methods for diagnosing and correcting problems in crystallographic models[2].
ReferencesReferences
- ↑ Li S, Finley J, Liu ZJ, Qiu SH, Chen H, Luan CH, Carson M, Tsao J, Johnson D, Lin G, Zhao J, Thomas W, Nagy LA, Sha B, DeLucas LJ, Wang BC, Luo M. Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain. J Biol Chem. 2002 Dec 13;277(50):48596-601. Epub 2002 Sep 7. PMID:12221106 doi:http://dx.doi.org/10.1074/jbc.M208512200
- ↑ Arendall WB 3rd, Tempel W, Richardson JS, Zhou W, Wang S, Davis IW, Liu ZJ, Rose JP, Carson WM, Luo M, Richardson DC, Wang BC. A test of enhancing model accuracy in high-throughput crystallography. J Struct Funct Genomics. 2005;6(1):1-11. PMID:15965733 doi:10.1007/s10969-005-3138-4