1jhj
Crystal structure of the APC10/Doc1 subunit of the human anaphase-promoting complex
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OverviewOverview
The anaphase-promoting complex (APC), or cyclosome, is a cell, cycle-regulated ubiquitin ligase that controls progression through mitosis, and the G1 phase of the cell cycle. The APC is composed of at least 11, subunits; no structure has been determined for any of these subunits. The, subunit APC10/DOC1, a one-domain protein consisting of 185 amino acids, has a conserved core (residues 22-161) that is homologous to domains found, in several other putative ubiquitin ligases and, therefore, may play a, role in ubiquitination reactions. Here we report the crystal structure of, human APC10 at 1.6 A resolution. The core of the protein is formed by a, beta-sandwich that adopts a jellyroll fold. Unexpectedly, this structure, is highly similar to ligand-binding domains of several bacterial and, eukaryotic proteins, such as galactose oxidase and coagulation factor Va, raising the possibility that APC10 may function by binding a yet, unidentified ligand. We further provide biochemical evidence that the, C-terminus of APC10 binds to CDC27/APC3, an APC subunit that contains, multiple tetratrico peptide repeats.
About this StructureAbout this Structure
1JHJ is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex., Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P, Nat Struct Biol. 2001 Sep;8(9):784-8. PMID:11524682
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