1kxc

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File:1kxc.gif


1kxc, resolution 3.1Å

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SINDBIS VIRUS CAPSID (N190K MUTANT), TETRAGONAL CRYSTAL FORM

OverviewOverview

Sindbis virus core protein (SCP) has been isolated from virus and, crystallized. The X-ray crystallographic structure showed that the, amino-terminal 113 residues appeared to be either disordered or truncated, during crystallization and that the carboxy-terminal residues 114 to 264, had a chymotrypsin-like structure. The carboxy-terminal residues 106 to, 264 and 106 to 266 of SCP have now been expressed in Escherichia coli., Most crystal forms of the truncated proteins were isomorphous with those, of the virally extracted protein. There are only small structural, differences between the truncated recombinant protein and the ordered part, of the wild-type virus-extracted protein. Hence, E. coli-expressed SCP can, be used to study proteolytic properties and the contribution of SCP to, ... [(full description)]

About this StructureAbout this Structure

1KXC is a [Single protein] structure of sequence from [Sindbis virus]. Structure known Active Site: TRI. Full crystallographic information is available from [OCA].

ReferenceReference

Structural analysis of Sindbis virus capsid mutants involving assembly and catalysis., Choi HK, Lee S, Zhang YP, McKinney BR, Wengler G, Rossmann MG, Kuhn RJ, J Mol Biol. 1996 Sep 20;262(2):151-67. PMID:8831786

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