1ba8
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THROMBIN INHIBITOR WITH A RIGID TRIPEPTIDYL ALDEHYDES
OverviewOverview
The crystal structures of three highly potent and selective low-molecular, weight rigid peptidyl aldehyde inhibitors complexed with thrombin have, been determined and refined to R values 0.152-0. 170 at 1.8-2.1 A, resolution. Since the selectivity of two of the inhibitors was >1600 with, respect to trypsin, the structures of trypsin-inhibited complexes of these, inhibitors were also determined (R = 0.142-0.157 at 1.9-2.1 A resolution)., The selectivity appears to reside in the inability of a benzenesulfonamide, group to bind at the equivalent of the D-enantiomorphic S3 site of, thrombin, which may be related to the lack of a 60-insertion loop in, trypsin. All the inhibitors have a novel lactam moiety at the P3 position, while the two with greatest trypsin selectivity have a ... [(full description)]
About this StructureAbout this Structure
1BA8 is a [Single protein] structure of sequence from [Homo sapiens] with NAG and PMS as [ligands]. Active as [Thrombin], with EC number [3.4.21.5]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
ReferenceReference
Highly selective mechanism-based thrombin inhibitors: structures of thrombin and trypsin inhibited with rigid peptidyl aldehydes., Krishnan R, Zhang E, Hakansson K, Arni RK, Tulinsky A, Lim-Wilby MS, Levy OE, Semple JE, Brunck TK, Biochemistry. 1998 Sep 1;37(35):12094-103. PMID:9724521
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