1d2b
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THE MMP-INHIBITORY, N-TERMINAL DOMAIN OF HUMAN TISSUE INHIBITOR OF METALLOPROTEINASES-1 (N-TIMP-1), SOLUTION NMR, 29 STRUCTURES
OverviewOverview
A high quality solution structure of the matrix metalloproteinase, inhibitory N-terminal domain of recombinant human tissue inhibitor of, metalloproteinases-1 (N-TIMP-1) has been determined. For the rigidly, packed residues, the average RMSD to the mean structure is 0. 57 A for the, backbone atoms and 1.00 A for all heavy atoms. Comparison of the solution, structure of free N-TIMP-1 with the crystal structure of TIMP-1 bound to, the catalytic domain of MMP-3 ( Gomis-R]uth et al., 1997 ) shows that the, structural core of the beta barrel flanked by helices is nearly unchanged, by the association with MMP-3, evident from a backbone RMSD of 1.15 A., However, clear differences in the conformation of the MMP-binding ridge of, free and MMP-bound TIMP-1 suggest induced fit throughout the ridge. The, MMP-dependent conformational changes in the ridge include a dramatic, bending of AB loop residues Glu28 through Leu34, moderate hinge bending of, the CD-loop about residues Ala65 and Cys70, and modest bending of the Cys1, through Pro6 segment. A large number of interresidue Nuclear Overhauser, enhancements (NOEs) augmented by stereospecific assignments, torsion, restraints, and dipolar couplings (an average of 18 non-trivial restraints, per residue) engender confidence in these structural inferences. A tight, cluster of three lysine residues and one arginine residue atop, beta-strands A and B, and identical among TIMP sequences, form the heart, of a highly conserved electropositive patch that may interact with anionic, components of the extracellular matrix.
DiseaseDisease
Known disease associated with this structure: Sorsby fundus dystrophy OMIM:[188826]
About this StructureAbout this Structure
1D2B is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
NMR structure of tissue inhibitor of metalloproteinases-1 implicates localized induced fit in recognition of matrix metalloproteinases., Wu B, Arumugam S, Gao G, Lee GI, Semenchenko V, Huang W, Brew K, Van Doren SR, J Mol Biol. 2000 Jan 14;295(2):257-68. PMID:10623524
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