5a3h
|
2-DEOXY-2-FLURO-B-D-CELLOBIOSYL/ENZYME INTERMEDIATE COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.8 ANGSTROMS RESOLUTION
OverviewOverview
The enzymatic hydrolysis of O-glycosidic linkages is one of the most, diverse and widespread reactions in nature and involves a classic, "textbook" enzyme mechanism. A multidisciplinary analysis of a, beta-glycoside hydrolase, the Cel5A from Bacillus agaradhaerens, is, presented in which the structures of each of the native, substrate, covalent-intermediate, and product complexes have been determined and, their interconversions analyzed kinetically, providing unprecedented, insights into the mechanism of this enzyme class. Substrate is bound in a, distorted 1S3 skew-boat conformation, thereby presenting the anomeric, carbon appropriately for nucleophilic attack as well as satisfying the, stereoelectronic requirements for an incipient oxocarbenium ion. Leaving, group departure results in the trapping of a covalent, alpha-glycosyl-enzyme intermediate in which the sugar adopts an, undistorted 4C1 conformation. Finally, hydrolysis of this intermediate, yields a product complex in which the sugar is bound in a partially, disordered mode, consistent with unfavorable interactions and low product, affinity.
About this StructureAbout this Structure
5A3H is a Single protein structure of sequence from Bacillus agaradhaerens with as ligand. Active as Cellulase, with EC number 3.2.1.4 Known structural/functional Sites: and . Full crystallographic information is available from OCA.
ReferenceReference
Snapshots along an enzymatic reaction coordinate: analysis of a retaining beta-glycoside hydrolase., Davies GJ, Mackenzie L, Varrot A, Dauter M, Brzozowski AM, Schulein M, Withers SG, Biochemistry. 1998 Aug 25;37(34):11707-13. PMID:9718293
Page seeded by OCA on Sun Feb 3 10:53:15 2008