2v08
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STRUCTURE OF WILD-TYPE PHORMIDIUM LAMINOSUM CYTOCHROME C6
OverviewOverview
Cytochrome c6A is a unique dithio-cytochrome of green algae and plants. It, has a very similar core structure to that of bacterial and algal, cytochromes c6 but is unable to fulfill the same function of transferring, electrons from cytochrome f to photosystem I. A key feature is that its, heme midpoint potential is more than 200 mV below that of cytochrome c6, despite having His and Met as axial heme-iron ligands. To identify the, molecular origins of the difference in potential, the structure of, cytochrome c6 from the cyanobacterium Phormidium laminosum has been, determined by X-ray crystallography and compared with the known structure, of cytochrome c6A. One salient difference of the heme pockets is that a, highly conserved Gln (Q51) in cytochrome c6 is replaced by Val (V52) in, c6A. Using protein film voltammetry, we found that swapping these residues, raised the c6A potential by +109 mV and decreased that of c6 by almost the, same extent, -100 mV. X-ray crystallography of the V52Q protein showed, that the Gln residue adopts the same configuration relative to the heme as, in cytochrome c6 and we propose that this stereochemistry destabilizes the, oxidized form of the heme. Consequently, replacement of Gln by Val was, probably a key step in the evolution of cytochrome c6A from cytochrome c6, inhibiting reduction by the cytochrome b6f complex and facilitating, establishment of a new function.
About this StructureAbout this Structure
2V08 is a Single protein structure of sequence from Phormidium laminosum with , , and as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
Modulation of Heme Redox Potential in the Cytochrome c(6) Family., Worrall JA, Schlarb-Ridley BG, Reda T, Marcaida MJ, Moorlen RJ, Wastl J, Hirst J, Bendall DS, Luisi BF, Howe CJ, J Am Chem Soc. 2007 Aug 1;129(30):9468-75. Epub 2007 Jul 11. PMID:17625855
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