Chitinase
Chitinases cleave the beta-1-4-glycosidic bond between the N-acetyl-D-glucosamine units of which chitin is comprised. Chitinases are present in plants, bacteria and fungi. The first chitinase structures were solved in 1994, from a bacterium (1ctn) and a plant (2hvm). A mechanism for chitin cleavage was proposed based on several structures and was later confirmed. The images at the left and at the right correspond to one representative Chitinase, i.e. the crystal structure of Chitinase I from Serratia marcescens (1edq).
3D Structures of Chitinase3D Structures of Chitinase
Update November 2011
Chitinase IChitinase I
3aro, 3b8s – VhChi residues 22-597 – Vibrio harveyi
3b9e - VhChi residues 22-597 (mutant)
1k85 – BcChi fibronectin domain – Bacillus circulans – NMR
1itx - BcChi catalytic domain
1ed7 – BcChi chitin-binding domain - NMR
1ll6, 1ll7 - CiChi residues 36-427 (mutant) – Coccicioides immitis
1d2k - CiChi residues 36-427
3n11 – BcChi – Bacillus cereus
3iwr, 2dkv – Chi residues 33-340 – Japanese rice
1edq, 1ctn - SmChi – Serratia marcescens
1x6l, 1rd6 – SmChi (mutant)
3g6l - BoChi residues 21-426 – Bionectria ochroleuca
Chitinase I binary complexChitinase I binary complex
3arp, 3arq, 3arr, 3ars, 3art, 3aru, 3arv, 3arw, 3arx, 3ary, 3arz – VhChi residues 22-597 + inhibitor
3as0, 3as1, 3as2, 3as3 – VhChi residues 22-597 (mutant) + inhibitor
3b9a, 3b9d - VhChi residues 22-597 + polysaccharide
2xvn, 2xuc – AfChi residues 29-307 + inhibitor – Aspergillus fumigatus
3n13, 3n15, 3n17, 3n18 – BcChi (mutant) + NAG
3n12 – BcChi + Zn
3n1a - BcChi (mutant) + inhibitor
1x6n - SmChi (mutant) + inhibitor
1ffq - SmChi + inhibitor
1nh6, 1k9t, 1ffr, 1eib, 1ehn - SmChi (mutant) + polysaccharide
2wk2, 2wly, 2wlz, 2wm0 – SmChi residues 2-520 + inhibitor
3g6m - BoChi residues 21-426 + caffeine
1waw, 1wb0 – hChi + polypeptide – human
1hkk, 1hkm – hChi + inhibitor
1ll4 - CiChi residues 36-427 + inhibitor
Chitinase IIChitinase II
1e15 - SmChi
1ogb, 1goi, 1e6p - SmChi (mutant)
1kfw – Chi catalytic domain – Arthrobacter
1dxj – Chi – Jack bean
Chitinase II binary complexChitinase II binary complex
1ogg, 1ur9 - SmChi (mutant) + inhibitor
1e6n - SmChi (mutant) + polysaccharide
1ur8, 1gpf, 1e6r - SmChi + inhibitor
1e6z – SmChi + intermediate
2a3a, 2a3b, 2a3c, 2a3e – AfChi + inhibitor
1w1p, 1w1t, 1w1v, 1w1y, 1o6i, 1h0g, 1h0i – SmChi + polypeptide
Chitinase IIIChitinase III
2dbt, 2wvu – SgChi residues 30-294 – Streptomyces griseus
1wvv - SgChi residues 30-294 (mutant)
2d49 – SgChi chitin-binding domain - NMR
2xtk – AfChi + inhibitor
Chitinase IVChitinase IV
3hbd, 3hbe, 3hbh - Chi catalytic domain – Norway spruce
Chitinase VChitinase V
3alf – tChi residues 26-377 – tobacco
Chitinase V binary complexChitinase V binary complex
3alg - tChi residues 26-377 (mutant) + NAG
3chc, 3chd, 3che, 3chf - AfChi + polypeptide
3ch9, 2iuz – AfChi + inhibitor
Chitinase VIIIChitinase VIII
2cjl - Chi residues 41-244 – Streptomyces coelicolor
ChitinaseChitinase
1wno, 1w9p - AfChi
2dsk - PfChi catalytic domain – Pyrococcus furiosus
3afb - PfChi catalytic domain (mutant)
1cns – Chi – Hordeum vulgare
2czn - PfChi chitin-binding domain – NMR
2cwr - PfChi chitin-binding domain
3ian – Chi (mutant) – Lactococcus lactis
3fxy - hChi catalytic domain
1lq0 - hChi residues 22-286
1guv - hChi residues 22-387
3fnd, 3co4 – Chi – Bacteroides thetaiotamicron
3cql – Chi – Carica papaya
2z37 - BjChi catalytic domain – Brassica juncea
2z39 – BjChi catalytic domain (mutant)
2uy2 – yChi residues 22-315 – yeast
Chitinase binary complexChitinase binary complex
3a4w, 3a4x – PfChi catalytic domain (mutant) + NAG
1lg1 - hChi residues 22-387 + chitobiose
1lg2 - hChi residues 22-387 + ethylene glycol
3fy1, 2ybt, 2ybu - hChi catalytic domain + inhibitor
1hki, 1hkj - hChi + inhibitor
2z38 - BjChi catalytic domain + Cl
2uy3, 2uy4, 2uy5 - yChi residues 22-315 + inhibitor
1w9v, 1w9u - AfChi + polypeptide