Nucleoprotein
Nucleoproteins (NP) are associated with DNA or RNA. NPs which contain RNA are called ribonucleoproteins (RNPs). The NP RNA-recognition motif is named RRM. The small nuclear ribonucleoproteins (snRNPs) are RNA-protein complexes which form part of the spliceosome. The heterogenous nuclear ribonucleoproteins (hnRNP) are RNA-protein complexes which associates with the splicing apparatus and prevent the folding of pre-mRNA. The images at the left and at the right correspond to one representative NP, i.e. the crystal structure of Nucleoprotein from Vesicular stomatitis Indiana virus (3pto).
3D Structures of Nucleoprotein3D Structures of Nucleoprotein
3ouo, 3ov9 – NP – Rift valley fever virus 3q7b, 3q7c, 3mwp, 3mwt – LvNP exonuclease domain – Lassa virus 3pto - VvNP – Vesicular stomatitis Indiana virus 2qvj – VvNP (mutant) 2k48 – NP N terminal – Andes virus - NMR 3hd4 – NP N terminal – Murine hepatitis virus 2wfs – IvNP – Cryo EM – Influenza virus 2q06, 2iqh – IvNP 2i8b – NP C terminal – Ebola virus – NMR 1yvr – XlNP Ro – Xenopus laevis 1wwf – MvNP P10 – Moloney murine leukemia virus – NMR 2c86 – BvNP N terminal – Avian infectious bronchitis virus 2ca1 – BvNP dimerization domain 2btl, 2bxx – BvNP RRM (mutant) 1n93, 1pp1 – NP P40 – Borna disease virus
Nucleoprotein binary complex
3mx2, 3mx5 – LvNP + nucleotide 3ptx, 3pu0, 3pu1, 3pu4, 2gic – VvNP + RNA 2wyy – VvNP + poly U – Cryo EM 3hhw – VvNP + phosphoprotein 3hhz - VvNP + phosphoprotein + RNA 2wj8 – NP + RNA – Human respiratory syncytial virus 2i91, 1yvp – XlNP Ro + RNA 2gtt – NP + RNA – Rabies virus 1wwg, 1wwd, 1wwe , 1u6p- MvNP P10 + RNA – NMR
Ribonucleoprotein
2lbw – yRNP subunit 2 residues 36-156 (mutant) – yeast – NMR 2lbx - yRNP subunit 2 residues 36-156 – NMR
Small nuclear ribonucleoprotein
2a3j - h-snRNP U1 A RRM – human - NMR 2l5i, 2l5j - h-snRNP U1 70kDa residues 131-151 – NMR 2vy4, 2vy5 - h-snRNP U11/U12 – NMR 2cqj - h-snRNP U3 S4 domain – NMR 1x4q - h-snRNP U4/U6 PWI domain - NMR 2vrd - h-snRNP U1 C zinc finger domain - NMR 2k3k – Dm-snRNP U1 A RRM1 – Drosophila melanogaster – NMR 2aym - Dm-snRNP U1 A 2b0g - Dm-snRNP U1 A RBD2 – NMR
Small nuclear ribonucleoprotein binary complex
3plu, 3plv – y-snRNP HINDI domain + ubiquitin-like modifier HUB1 2y9a, 2y9b, 2y9c, 2y9d – h-snRNP U4 core domain 3p49 - h-snRNP A RRM + glycine riboswitch RNA 3pgw, 3mum, 3mur, 3mut, 3muv, 3egz - h-snRNP U1 A + DNA riboswitch 3mxh, 3irw - h-snRNP U1 A (mutant) + DNA riboswitch 3k0j - h-snRNP U1 A (mutant) + RNA 3iin - h-snRNP U1 A (mutant) + group I intron + DNA 3b02, 3b03, 3b04 - h-snRNP U1 A RRM1 + group I intron + RNA 1zzn - h-snRNP U1 A RRM1 (mutant) + group I intron + RNA 3l3c, 3g8s, 3g8t, 3g96, 3g9c, 2nz4 - h-snRNP U1 A (mutant) + GLMS ribozyme + RNA 3hhn - h-snRNP U1 A (mutant) + class I ligase ribozyme 2oih, 2oj3 - h-snRNP U1 A + HDV ribozyme 3cul, 3cun - h-snRNP U1 A (mutant) + aminoacyl-tRNA synthetase ribozyme 3cw1 - h-snRNP U1 in spliceosome 2ozb - h-snRNP U4/U6 + NHP2-like protein + RNA 2bn5 - Dm-snRNP U1 70kDa + PSI B box – NMR
Heterogenous nuclear ribonucleoprotein
3s01 – m-hnRNP L RRM3 domain – mouse 2e5i - m-hnRNP L RRM – NMR 2db1 - m-hnRNP F RRM – NMR 1x4b - h-hnRNP A2/B1 RRM – NMR 1txp - h-hnRNP C oligomerization domain – NMR 1wf2 - h-hnRNP C1/C2 RRM – NMR 2hgl, 2hgm, 2hgn - h-hnRNP F QRRM – NMR 1wg5, 1wez - h-hnRNP H RRM – NMR 2ad9, 2adb, 2adc - h-hnRNP I – NMR 1zzi, 1zzj - h-hnRNP K KH domain + DNA 1zzk - h-hnRNP K KH domain 2do0, 2dh9, 2dgv - h-hnRNP M RRM – NMR 2dgu - h-hnRNP Q RRM – NMR 2dk2 - h-hnRNP R RRM – NMR
Heterogenous nuclear ribonucleoprotein binary complex
2kfy, 2kg0, 2kg1 - h-hnRNP F QRRM + DNA – NMR 2z5n, 2z5o, 2ot8 - h-hnRNP D0 C terminal + transportin-1 1u1k, 1u1l, 1u1m, 1u1n, 1u1o, 1u1p, 1u1q, 1u1r - h-hnRNP A1 + DNA 1wtb, 1x0f - h-hnRNP D0 RRM + DNA – NMR