2ns4
Solution structure of a Beta-Hairpin Peptidomimetic Inhibitor of the BIV Tat-Tar Interaction
|
OverviewOverview
The Tat protein of immunodeficiency viruses is the main activator of viral, gene expression. By binding specifically to its cognate site, the, transactivator response element (TAR), Tat mediates a strong induction of, the production of all viral transcripts. In seeking a new chemical, solution to inhibiting viral protein-RNA interactions, we recently, identified inhibitors of the viral Tat protein from the bovine, immunodeficiency virus (BIV) using conformationally constrained, beta-hairpin peptidomimetics. We identified a micromolar ligand, called, BIV2, and the structure of its complex with BIV TAR was determined by NMR., In this work, we demonstrate that this chemistry can rapidly yield highly, potent and selective ligands. On the basis of the structure, we, synthesized and assayed libraries of mutant peptidomimetics. Remarkably, we were able in just a few rounds of design and synthesis to discover, nanomolar inhibitors of the Tat-TAR interaction in BIV that selectively, bind the BIV TAR RNA compared to RNA structures as closely related as the, HIV-1 TAR or RRE elements. The molecular recognition principles developed, in this study have been exploited in discovering related peptidomimetic, inhibitors of the Tat-TAR interaction in HIV-1.
About this StructureAbout this Structure
2NS4 is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structure-guided peptidomimetic design leads to nanomolar beta-hairpin inhibitors of the Tat-TAR interaction of bovine immunodeficiency virus., Athanassiou Z, Patora K, Dias RL, Moehle K, Robinson JA, Varani G, Biochemistry. 2007 Jan 23;46(3):741-51. PMID:17223695
Page seeded by OCA on Tue Jan 29 20:58:51 2008