2ijg
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Crystal Structure of cryptochrome 3 from Arabidopsis thaliana
OverviewOverview
Cryptochromes use near-UV/blue light to regulate a variety of growth and, adaptive process. Recent biochemical studies demonstrate that the, Cryptochrome-Drosophila, Arabidopsis, Synechocystis, Human (Cry-DASH), subfamily of cryptochromes have photolyase activity exclusively for, single-stranded cyclobutane pyrimidine dimer (CPD)-containing DNA, substrate [Selby C, Sancar A (2006) Proc Natl Acad Sci USA, 103:17696-17700]. The crystal structure of cryptochrome 3 from Arabidopsis, thaliana (At-Cry3), a member of the Cry-DASH proteins, at 2.1 A, resolution, reveals that both the light-harvesting cofactor, 5,10-methenyl-tetrahydrofolyl-polyglutamate (MTHF) and the catalytic, cofactor flavin adenine dinucleotide (FAD) are noncovalently bound to the, protein. The residues responsible for binding of MTHF in At-Cry3 are not, conserved in Escherichia coli photolyase but are strongly conserved in the, Cry-DASH subfamily of cryptochromes. The distance and orientation between, MTHF and flavin adenine dinucleotide in At-Cry3 is similar to that of E., coli photolyase, in conjunction with the presence of electron transfer, chain, suggesting the conservation of redox activity in At-Cry3. Two amino, acid substitutions and the penetration of three charged side chains into, the CPD-binding cavity in At-Cry3 alter the hydrophobic environment that, is accommodating the hydrophobic sugar ring and thymine base moieties in, class I CPD photolyases. These changes most likely make CPD binding less, energetically favorable and, hence, insufficient to compete with pairing, and stacking interactions between the CPD and the duplex DNA substrate., Thus, Cry-DASH subfamily proteins may be unable to stabilize CPD flipped, out from the duplex DNA substrate but may be able to preserve the DNA, repair activity toward single-stranded CPD-containing DNA substrate.
About this StructureAbout this Structure
2IJG is a Single protein structure of sequence from Arabidopsis thaliana with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of cryptochrome 3 from Arabidopsis thaliana and its implications for photolyase activity., Huang Y, Baxter R, Smith BS, Partch CL, Colbert CL, Deisenhofer J, Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17701-6. Epub 2006 Nov 13. PMID:17101984
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