2g3o
The 2.1A crystal structure of copGFP
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OverviewOverview
The green fluorescent protein (avGFP), its variants, and the closely, related GFP-like proteins are characterized structurally by a cyclic, tri-peptide chromophore located centrally within a conserved beta-can, fold. Traditionally, these GFP family members have been isolated from the, Cnidaria although recently, distantly related GFP-like proteins from the, Bilateria, a sister group of the Cnidaria have been described, although no, representative structure from this phylum has been reported to date. We, have determined to 2.1A resolution the crystal structure of copGFP, a, representative GFP-like protein from a copepod, a member of the Bilateria., The structure of copGFP revealed that, despite sharing only 19% sequence, identity with GFP, the tri-peptide chromophore (Gly57-Tyr58-Gly59) of, copGFP adopted a cis coplanar conformation within the conserved beta-can, fold. However, the immediate environment surrounding the chromophore of, copGFP was markedly atypical when compared to other members of the, GFP-superfamily, with a large network of bulky residues observed to, surround the chromophore. Arg87 and Glu222 (GFP numbering 96 and 222), the, only two residues conserved between copGFP, GFP and GFP-like proteins are, involved in autocatalytic genesis of the chromophore. Accordingly, the, copGFP structure provides an alternative platform for the development of a, new suite of fluorescent protein tools. Moreover, the structure suggests, that the autocatalytic genesis of the chromophore is remarkably tolerant, to a high degree of sequence and structural variation within the beta-can, fold of the GFP superfamily.
About this StructureAbout this Structure
2G3O is a Single protein structure of sequence from Pontellina plumata. Full crystallographic information is available from OCA.
ReferenceReference
The 2.1A crystal structure of copGFP, a representative member of the copepod clade within the green fluorescent protein superfamily., Wilmann PG, Battad J, Petersen J, Wilce MC, Dove S, Devenish RJ, Prescott M, Rossjohn J, J Mol Biol. 2006 Jun 16;359(4):890-900. Epub 2006 Apr 25. PMID:16697009
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