2dsk

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Crystal structure of catalytic domain of hyperthermophilic chitinase from Pyrococcus furiosus

File:2dsk.jpg


2dsk, resolution 1.50Å

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OverviewOverview

The crystal structure of the catalytic domain of a chitinase from the, hyperthermophilic archaeon Pyrococcus furiosus (AD2(PF-ChiA)) has been, determined at 1.5 A resolution. This is the first structure of the, catalytic domain of an archaeal chitinase. The overall structure of, AD2(PF-ChiA) is a TIM-barrel fold with a tunnel-like active site that is a, common feature of family 18 chitinases. Although the catalytic residues, (Asp522, Asp524 and Glu526) are conserved, comparison of the conserved, residues and structures with those of other homologous chitinases, indicates that the catalytic mechanism of PF-ChiA is different from that, of family 18 chitinases.

About this StructureAbout this Structure

2DSK is a Single protein structure of sequence from Pyrococcus furiosus with and as ligands. Active as Chitinase, with EC number 3.2.1.14 Full crystallographic information is available from OCA.

ReferenceReference

Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus., Nakamura T, Mine S, Hagihara Y, Ishikawa K, Uegaki K, Acta Crystallograph Sect F Struct Biol Cryst Commun. 2007 Jan 1;63(Pt, 1):7-11. Epub 2006 Dec 16. PMID:17183162

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