2cwq
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Crystal structure of conserved protein TTHA0727 from Thermus thermophilus HB8
OverviewOverview
TTHA0727 is a conserved hypothetical protein from Thermus thermophilus, HB8, with a molecular mass of 12.6 kDa. TTHA0727 belongs to the, carboxymuconolactone decarboxylase (CMD) family (Pfam 02627). A sequence, comparison with its homologs suggested that TTHA0727 is a distinct protein, from alkylhydroperoxidase AhpD and gamma-carboxymuconolactone, decarboxylase in the CMD family. Here we report the 1.9 A crystal, structure of TTHA0727 (PDB ID: 2CWQ) determined by the multiwavelength, anomalous dispersion method. The TTHA0727 monomer structure consists of, seven alpha-helices (alpha1-alpha7) and one short 3(10)-helix. The crystal, structure and the analytical ultracentrifugation revealed that TTHA0727, forms a hexameric ring structure in solution. The electrostatic potential, distribution on the solvent-accessible surface of the TTHA0727 hexamer, showed that positively charged regions exist on the side of the ring, structure, suggesting that TTHA0727 interacts with some negatively charged, molecules. A structural homology search revealed that the structure of, three alpha-helices (alpha4-alpha6) is remarkably conserved, suggesting, that it is the common structural motif for the CMD family proteins. In, addition, the nine residues of the N-terminal tag bound to the cleft, region between alpha1 and alpha3 in chains A and B of TTHA0727, implying, that this region is the putative binding/active site for some small, molecules.
About this StructureAbout this Structure
2CWQ is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the conserved protein TTHA0727 from Thermus thermophilus HB8 at 1.9 A resolution: A CMD family member distinct from carboxymuconolactone decarboxylase (CMD) and AhpD., Ito K, Arai R, Fusatomi E, Kamo-Uchikubo T, Kawaguchi S, Akasaka R, Terada T, Kuramitsu S, Shirouzu M, Yokoyama S, Protein Sci. 2006 May;15(5):1187-92. Epub 2006 Apr 5. PMID:16597838
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- Single protein
- Thermus thermophilus
- Arai, R.
- Fusatomi, E.
- Ito, K.
- Kamo-Uchikubo, T.
- Kawaguchi, S.
- RSGI, RIKEN.Structural.Genomics/Proteomics.Initiative.
- Shirouzu, M.
- Terada, T.
- Yokoyama, S.
- All alpha
- Conserved hypothetical protein
- National project on protein structural and functional analyses
- Nppsfa
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomics