2a6m
Crystal Structure of the ISHp608 Transposase
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OverviewOverview
Many bacteria harbor simple transposable elements termed insertion, sequences (IS). In Helicobacter pylori, the chimeric IS605 family elements, are particularly interesting due to their proximity to genes encoding, gastric epithelial invasion factors. Protein sequences of IS605, transposases do not bear the hallmarks of other well-characterized, transposases. We have solved the crystal structure of full-length, transposase (TnpA) of a representative member, ISHp608. Structurally, TnpA, does not resemble any characterized transposase; rather, it is related to, rolling circle replication (RCR) proteins. Consistent with RCR, Mg2+ and a, conserved tyrosine, Tyr127, are essential for DNA nicking and the, formation of a covalent intermediate between TnpA and DNA. TnpA is, dimeric, contains two shared active sites, and binds two DNA stem loops, representing the conserved inverted repeats near each end of ISHp608. The, cocrystal structure with stem-loop DNA illustrates how this family of, transposases specifically recognizes and pairs ends, necessary steps, during transposition.
About this StructureAbout this Structure
2A6M is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.
ReferenceReference
Active site sharing and subterminal hairpin recognition in a new class of DNA transposases., Ronning DR, Guynet C, Ton-Hoang B, Perez ZN, Ghirlando R, Chandler M, Dyda F, Mol Cell. 2005 Oct 7;20(1):143-54. PMID:16209952
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