2oe8
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1.8 A X-ray crystal structure of Apramycin complex with RNA fragment GGGCGUCGCUAGUACC/CGGUACUAAAAGUCGCC containing the human ribosomal decoding A site: RNA construct with 5'-overhang
OverviewOverview
Aminoglycoside antibiotics bind specifically to the bacterial ribosomal, decoding-site RNA and thereby interfere with fidelity but not efficiency, of translation. Apramycin stands out among aminoglycosides for its, mechanism of action which is based on blocking translocation and its, ability to bind also to the eukaryotic decoding site despite differences, in key residues required for apramycin recognition by the bacterial, target. To elucidate molecular recognition of the eukaryotic decoding site, by apramycin we have determined the crystal structure of an, oligoribonucleotide containing the human sequence free and in complex with, the antibiotic at 1.5 A resolution. The drug binds in the deep groove of, the RNA which forms a continuously stacked helix comprising non-canonical, CA and GA base pairs and a bulged-out adenine. The binding mode of, apramycin at the human decoding-site RNA is distinct from aminoglycoside, recognition of the bacterial target, suggesting a molecular basis for the, actions of apramycin in eukaryotes and bacteria.
About this StructureAbout this Structure
2OE8 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Apramycin recognition by the human ribosomal decoding site., Hermann T, Tereshko V, Skripkin E, Patel DJ, Blood Cells Mol Dis. 2007 Jan 26;. PMID:17258916
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