2ihk
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crystal structure of multifunctional sialyltransferase from pasteurella multocida with CMP-3F(equatorial)-Neu5Ac bound
OverviewOverview
Sialyltransferases are key enzymes involved in the biosynthesis of, biologically and pathologically important sialic acid-containing molecules, in nature. Binary X-ray crystal structures of a multifunctional, Pasteurella multocida sialyltransferase (Delta24PmST1) with a donor, analogue CMP-3F(a)Neu5Ac or CMP-3F(e)Neu5Ac were determined at 2.0 and 1.9, A resolutions, respectively. Ternary X-ray structures of the protein in, complex with CMP or a donor analogue CMP-3F(a)Neu5Ac and an acceptor, lactose have been determined at 2.0 and 2.27 A resolutions, respectively., This represents the first sialyltransferase structure and the first, GT-B-type glycosyltransferase structure that is bound to both a donor, analogue and an acceptor simultaneously. The four structures presented, here reveal that binding of the nucleotide-activated donor sugar causes a, buried tryptophan to flip out of the protein core to interact with the, donor sugar and helps define the acceptor sugar binding site., Additionally, key amino acid residues involved in the catalysis have been, identified. Structural and kinetic data support a direct displacement, mechanism involving an oxocarbenium ion-like transition state assisted, with Asp141 serving as a general base to activate the acceptor hydroxyl, group.
About this StructureAbout this Structure
2IHK is a Single protein structure of sequence from Pasteurella multocida with as ligand. Active as Beta-galactoside alpha-2,3-sialyltransferase, with EC number 2.4.99.4 Full crystallographic information is available from OCA.
ReferenceReference
Crystal Structures of Pasteurella multocida Sialyltransferase Complexes with Acceptor and Donor Analogues Reveal Substrate Binding Sites and Catalytic Mechanism(,)., Ni L, Chokhawala HA, Cao H, Henning R, Ng L, Huang S, Yu H, Chen X, Fisher AJ, Biochemistry. 2007 May 29;46(21):6288-98. Epub 2007 May 8. PMID:17487984
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