1wq2
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Neutron Crystal Structure Of Dissimilatory Sulfite Reductase D (DsrD)
OverviewOverview
Dissimilatory sulfite reductase D (DsrD) from Desulfovibrio vulgaris has, been crystallized for a neutron diffraction study. The initial crystals, obtained were too small for the neutron experiment. In order to obtain a, larger crystal (>1 mm3), a combination of two techniques was developed to, determine the optimum crystallization conditions: a crystallization phase, diagram was obtained, followed by crystal-quality assessment via X-ray, diffraction. Using conditions determined in this manner, a large single, crystal (1.7 mm3) of DsrD protein was subsequently grown in D(2)O solution, by the macroseeding technique. A neutron diffraction experiment was, carried out using the BIX-3 diffractometer at the Japan Atomic Energy, Research Institute (JAERI), collecting data to 2.4 A resolution from an, optimized crystal.
About this StructureAbout this Structure
1WQ2 is a Single protein structure of sequence from Desulfovibrio vulgaris with and as ligands. Full crystallographic information is available from OCA.
ReferenceReference
Crystallization and preliminary neutron analysis of the dissimilatory sulfite reductase D (DsrD) protein from the sulfate-reducing bacterium Desulfovibrio vulgaris., Chatake T, Mizuno N, Voordouw G, Higuchi Y, Arai S, Tanaka I, Niimura N, Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2306-9. Epub 2003, Nov 27. PMID:14646103
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