Colicin E3 is a type of Colicin, a bacteriocin made by E. Coli which acts against other nearby E. Coli to kill them with its 16s rRNase activity; it digests the 16s ribosomal subunit, ultimately leading to the death of the cell.
Synthesis and releaseSynthesis and release
When colicin E3 is synthesised, it binds its immunity protein, Im3, to its cytotoxic domain in the cytoplasm. This prevents its RNase activity from occurring in the cell in which it is made, so it does not kill it. It is released as this complex into the surrounding area, and Im3 is only released upon binding to a target cell.
Mechanism of uptakeMechanism of uptake
|
|
| 1ujw, resolution 2.75Å ()
|
| Ligands:
|
, , , ,
|
| Related:
|
1nqe, 1jch
|
| Structural annotation:
|
| Resources:
|
CATH : 1Ujwa01, 1Ujwa02, 1Ujwb00
InterPro : Ipr000531, Ipr010917, Ipr010916, Ipr012910, Ipr010101, Ipr016128, Ipr009105, Ipr003058
Pfam : PF00593, PF07715, PF11570
SCOP : d1ujwa_, d1ujwb_
|
|
|
|
| Resources:
|
FirstGlance, OCA, PDBsum, RCSB
|
| Coordinates:
|
save as pdb, mmCIF, xml
|
ColE3 initially binds to the BtuB vitamin B12 receptor. Formation of this complex leads to the unfolding of the N terminal receptor binding coiled-coil domain of ColE3. [1]
The structure shows the complex formed between BtuB and the ColE3 translocation domain [2]
Killing ActivitiesKilling Activities
|
|
| 2xfz, resolution 3.20Å ()
|
| Ligands:
|
,
|
| Non-Standard Residues:
|
|
| Related:
|
2xg0, 2xg1, 2xg2, 1ibk, 1ibl, 1jch, 2uuc, 2wdk, 2j02, 1j5e, 2wrq, 2j00, 1hnx, 2b9o, 2x9t, 2wh3, 2uub, 1vov, 1emi, 1pnx, 2v46, 2vqf, 2wh1, 1n32, 2wrn, 2f4v, 2jl5, 1twt, 1e44, 1qzc, 2uxd, 1fka, 1fjg, 2uxc, 1n36, 1hnw, 1l1u, 1hr0, 2v48, 2b64, 1xmq, 2b9m, 1pn8, 2wdg, 1xnr, 1hnz, 1dv4, 1rss, 2wdh, 1jgq, 1i94, 1i96, 2uu9, 1pn7, 1eg0, 1ibm, 1ujw, 2uxb, 2vqe, 1g1x, 2uua, 1i95, 1xnq, 1qd7, 1gix, 1jgo, 1jgp, 2x9r, 1n34, 1n33, 1pns, 2jl7, 1yl4, 2wdm, 1xmo, 1i97
|
| Structural annotation:
|
| Resources:
|
CATH : 2Xfzb01, 2Xfzb02, 2Xfzc01, 2Xfzc02, 2Xfzd01, 2Xfzd02, 2Xfze01, 2Xfze02, 2Xfzf00, 2Xfzg00, 2Xfzh01, 2Xfzh02, 2Xfzi00, 2Xfzk00, 2Xfzl00, 2Xfzm01, 2Xfzm02, 2Xfzn00, 2Xfzo00, 2Xfzr00, 2Xfzs00, 2Xfzt00, 2Xfzy00
InterPro : Ipr001865, Ipr005706, Ipr018130, Ipr023591, Ipr001351, Ipr004044, Ipr004087, Ipr005704, Ipr009019, Ipr015946, Ipr018280, Ipr001912, Ipr002942, Ipr005709, Ipr018079, Ipr022801, Ipr000851, Ipr005324, Ipr005712, Ipr013810, Ipr014720, Ipr014721, Ipr018192, Ipr020568, Ipr000529, Ipr014717, Ipr020814, Ipr020815, Ipr000235, Ipr005717, Ipr020606, Ipr023798, Ipr000630, Ipr000754, Ipr020574, Ipr023035, Ipr001848, Ipr018268, Ipr027486, Ipr001971, Ipr018102, Ipr019981, Ipr005679, Ipr006032, Ipr012340, Ipr001892, Ipr010979, Ipr018269, Ipr019980, Ipr027437, Ipr001209, Ipr018271, Ipr023053, Ipr000589, Ipr005290, Ipr009068, Ipr000307, Ipr023803, Ipr000266, Ipr019979, Ipr019984, Ipr001648, Ipr018275, Ipr002222, Ipr005732, Ipr020934, Ipr023575, Ipr002583, Ipr009105
Pfam : PF00318, PF00189, PF07650, PF00163, PF01479, PF00333, PF03719, PF01250, PF00177, PF00410, PF00380, PF00338, PF00411, PF00164, PF00416, PF00253, PF00312, PF00886, PF00366, PF01084, PF00203, PF01649, PF09000
|
|
| Resources:
|
FirstGlance, OCA, RCSB, PDBsum
|
| Coordinates:
|
save as pdb, mmCIF, xml
|
ReferencesReferences
- ↑ Masi M, Vuong P, Humbard M, Malone K, Misra R. Initial steps of colicin E1 import across the outer membrane of Escherichia coli. J Bacteriol. 2007 Apr;189(7):2667-76. Epub 2007 Feb 2. PMID:17277071 doi:10.1128/JB.01448-06
- ↑ Kurisu G, Zakharov SD, Zhalnina MV, Bano S, Eroukova VY, Rokitskaya TI, Antonenko YN, Wiener MC, Cramer WA. The structure of BtuB with bound colicin E3 R-domain implies a translocon. Nat Struct Biol. 2003 Nov;10(11):948-54. Epub 2003 Oct 5. PMID:14528295 doi:10.1038/nsb997
