Subtilisin

Crystal Structure of Subtilisin 2z2x

Subtilisin is a serine protease. It is widely used commercially in detergents. A 77 amino acid propeptide is cleaved from the N-terminus of pro-Sub to create the mature active enzyme. The images at the left and at the right correspond to one representative Subtilisin, i.e. the crystal structure of native mature subtilisin from Thermococcus kodakarensis (2z2x).

3D Structures of Subtilisin3D Structures of Subtilisin

Native mature subtilisinNative mature subtilisin

2z2x – TkSub - Thermococcus kodakarensis 1sca, 1scb, 1sbc – BsSub - Bacillus subtilis (Subtilisin Carlsberg) 1gns, 1sup, 1st2, 1st1, 1s01, 1sbt, 2sbt – BaSub - Bacillus amyloliquefaciens 1iav, 1gci, 1jea, 1st3 – BlSub - Bacillus lentus (Subtilisin Savinase) 1ea7, 3d43, 2ixt – spehricase – Sub from Bacillus sphaericus

Mutant mature subtilisinMutant mature subtilisin

3f49, 3co0, 2zrq – TkSubb (mutant) 1gnv, 1dui, 1sue, 1a2q, 1aqn, 1sua, 1au9, 1ak9, 1yja, 1yjb, 1yjc, 1sbh, 1sbi, 1sub, 1suc, 1sud, 1s02 – BaSub (mutant) 1ndu, 1q5p, 1c9j, 1c9m, 1c9n – BlSub (mutant)

Native unautoprocessed subtilisin (pro-Sub)Native unautoprocessed subtilisin (pro-Sub)

2z2z, 2e1p – pro-TkSub

Mutant unautoprocessed subtilisinMutant unautoprocessed subtilisin

2zwo, 2zwp – pro-TKSub

Mature subtilisin complexed with its propeptideMature subtilisin complexed with its propeptide

3cnq – BaSub 1spb – BaSub (mutant) 3a3n, 3a3o, 3a3p, 2z56, 2z57, 2z58, 2z2y, 2z30 – TkSub 1scj – BsSub

Subtilisin complexed with polypeptide inhibitorSubtilisin complexed with polypeptide inhibitor

1y1k, 1y33, 1y34, 1y3b, 1y3c, 1y3d, 1y3f, 1y48, 1y4a, 1y4d, 1tm3, 1tm4, 1tm1, 1lw6 – BaSub+ chymotripsin inhibitor 2 3sic, 5sic, 2sic – BaSub+Streptomyces inhibitor SSI 1yu6 – BsSub+ovomucoid 1v5i – BaSubb+POIA1 peptide 1tk2, 1ndq – BlSub+gramicidin S 1r0r – BsSub+ovomucoid 1oyv – BsSub+tomato inhibitor II 1sbn, 1sib, 2sec, 1sni, 1sce – BsSub+Elgin C 1mee - BpSub+Elgin C – Bacillus pumilus

Subtilisin complexed with inhibitorSubtilisin complexed with inhibitor

3bgo – BaSub+azide 1c3l – BsSub+Xe 1be8 – BsSub+cinnamoyl 1be6 - BsSub+cinnamoyl+acetonitrile 1bfk , 1scd– BsSub+acetonitrile 1bfu, 1af4 – BsSub+dioxane 1av7, 1avt, 3vsb, 1vsb – BsSub+boronic acid derivatives 1scn – BsSub+carbamate derivative 1bh6 – Sub DY+benzyloxycarbonyl-ala-pro-phe-chloromethyl ketone –Bacillus licheniformis

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Alexander Berchansky, Michal Harel, Joel L. Sussman