2ptg
Crystal structure of Eimeria tenella enoyl reductase
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OverviewOverview
SUMMARYApicomplexan parasites of the genus Eimeria are the major causative, agent of avian coccidiosis, leading to high economic losses in the poultry, industry. Recent results show that Eimeria tenella harbours an apicoplast, organelle, and that a key biosynthetic enzyme, enoyl reductase, is located, in this organelle. In related parasites, enoyl reductase is one component, of a type II fatty acid synthase (FAS) and has proven to be an attractive, target for antimicrobial compounds. We cloned and expressed the mature, form of E. tenella enoyl reductase (EtENR) for biochemical and structural, studies. Recombinant EtENR exhibits NADH-dependent enoyl reductase, activity and is inhibited by triclosan with an IC50 value of 60 nm. The, crystal structure of EtENR reveals overall similarity with other ENR, enzymes; however, the active site of EtENR is unoccupied, a state rarely, observed in other ENR structures. Furthermore, the position of the central, beta-sheet appears to block NADH binding and would require significant, movement to allow NADH binding, a feature not previously seen in the ENR, family. We analysed the E. tenella genomic database for orthologues of, well-characterized bacterial and apicomplexan FAS enzymes and identified 6, additional genes, suggesting that E. tenella contains a type II FAS, capable of synthesizing saturated, but not unsaturated, fatty acids., Interestingly, we also identified sequences that appear to encode, multifunctional type I FAS enzymes, a feature also observed in Toxoplasma, gondii, highlighting the similarity between these apicomplexan parasites.
About this StructureAbout this Structure
2PTG is a Single protein structure of sequence from Eimeria tenella. Active as [acyl-carrier-protein_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number 1.3.1.9 Full crystallographic information is available from OCA.
ReferenceReference
Type I and type II fatty acid biosynthesis in Eimeria tenella: enoyl reductase activity and structure., Lu JZ, Muench SP, Allary M, Campbell S, Roberts CW, Mui E, McLeod RL, Rice DW, Prigge ST, Parasitology. 2007 Aug 13;:1-14. PMID:17697396 [[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
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