2osr
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NMR Structure of RRM-2 of Yeast NPL3 Protein
OverviewOverview
Yeast Npl3 is homologous to SR proteins in higher eukaryotes, a family of, RNA-binding proteins that have multiple essential roles in RNA metabolism., This protein competes with 3'-end processing factors for binding to the, nascent RNA, protecting the transcript from premature termination and, coordinating transcription termination and the packaging of the fully, processed transcript for export. The NMR structure of its RNA-binding, domain shows two unusually compact RNA recognition motifs (RRMs), and, identifies the RNA recognition surface in Npl3. Biochemical and NMR, studies identify a class of G+U-rich RNA sequences with high specificity, for this protein. The protein binds to RNA and forms a single globular, structure, but the two RRMs of Npl3 are not equivalent, with the second, domain forming much stronger interactions with G+U-rich RNA sequences that, occur independently of the interaction of the first RRM. The specific, binding to G+U-rich RNAs observed for the two RRMs of Npl3 is masked in, the full-length protein by a much stronger but non-sequence-specific, RNA-binding activity residing outside of its RRMs. The preference of Npl3, for G+U-rich sequences supports the model for its function in regulating, recognition of 3'-end processing sites through competition with the Rna15, (yeast analog of human CstF-64 protein) subunit of the processing complex.
About this StructureAbout this Structure
2OSR is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals., Deka P, Bucheli ME, Moore C, Buratowski S, Varani G, J Mol Biol. 2008 Jan 4;375(1):136-50. Epub 2007 Sep 16. PMID:18022637
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