1e7d
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ENDONUCLEASE VII (ENDOVII) FROM PHAGE T4
OverviewOverview
The structure of the N62D mutant of the junction-resolving endonuclease, VII (EndoVII) from phage T4 has been refined at 1.3 A, and a second, wild-type crystal form solved and refined at 2.8 A resolution. Comparison, of the mutant with the wild-type protein structure in two different, crystal environments reveals considerable conformational flexibility at, the dimer level affecting the substrate-binding cleft, the dimerization, interface and the orientation of the C-terminal domains. The opening of, the DNA-binding cleft, the orientation of the C-terminal domains relative, to the central dimerization domain as well as the relative positioning of, helices in the dimerization interface appear to be sensitive to the, crystal packing environment. The highly unexpected rearrangement within, ... [(full description)]
About this StructureAbout this Structure
1E7D is a [Single protein] structure of sequence from [Enterobacteria phage t2] with ZN, CA and CL as [ligands]. Structure known Active Sites: AC1, AC2, AC3 and AC4. Full crystallographic information is available from [OCA].
ReferenceReference
Conformational flexibility in T4 endonuclease VII revealed by crystallography: implications for substrate binding and cleavage., Raaijmakers H, Toro I, Birkenbihl R, Kemper B, Suck D, J Mol Biol. 2001 Apr 27;308(2):311-23. PMID:11327769
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