1uw4
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THE STRUCTURAL BASIS OF THE INTERACTION BETWEEN NONSENSE MEDIATED DECAY FACTORS UPF2 AND UPF3
OverviewOverview
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism by which, eukaryotic cells detect and degrade transcripts containing premature, termination codons. Three 'up-frameshift' proteins, UPF1, UPF2 and UPF3, are essential for this process in organisms ranging from yeast to human., We present a crystal structure at a resolution of 1.95 A of the complex, between the interacting domains of human UPF2 and UPF3b, which are, respectively, a MIF4G (middle portion of eIF4G) domain and an RNP domain, (ribonucleoprotein-type RNA-binding domain). The protein-protein interface, is mediated by highly conserved charged residues in UPF2 and UPF3b and, involves the beta-sheet surface of the UPF3b RNP domain, which is, generally used by these domains to bind nucleic acids. We show that the, UPF3b ... [(full description)]
About this StructureAbout this Structure
1UW4 is a [Protein complex] structure of sequences from [Homo sapiens] with BME as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
ReferenceReference
The structural basis for the interaction between nonsense-mediated mRNA decay factors UPF2 and UPF3., Kadlec J, Izaurralde E, Cusack S, Nat Struct Mol Biol. 2004 Apr;11(4):330-7. Epub 2004 Mar 7. PMID:15004547
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