1ohh
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BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH THE INHIBITOR PROTEIN IF1
OverviewOverview
In mitochondria, the hydrolytic activity of ATP synthase is prevented by, an inhibitor protein, IF1. The active bovine protein (84 amino acids) is, an alpha-helical dimer with monomers associated via an antiparallel, alpha-helical coiled coil composed of residues 49-81. The N-terminal, inhibitory sequences in the active dimer bind to two F1-ATPases in the, presence of ATP. In the crystal structure of the F1-IF1 complex at 2.8 A, resolution, residues 1-37 of IF1 bind in the alpha(DP)-beta(DP) interface, of F1-ATPase, and also contact the central gamma subunit. The inhibitor, opens the catalytic interface between the alpha(DP) and beta(DP) subunits, relative to previous structures. The presence of ATP in the catalytic site, of the beta(DP) subunit implies that the inhibited state represents a, pre-hydrolysis step on the catalytic pathway of the enzyme.
About this StructureAbout this Structure
1OHH is a Protein complex structure of sequences from Bos taurus with MG and ANP as ligands. Active as Transferred entry: 3.6.3.14, with EC number 3.6.1.34 Known structural/functional Site: . Full crystallographic information is available from OCA.
ReferenceReference
The structure of bovine F1-ATPase in complex with its regulatory protein IF1., Cabezon E, Montgomery MG, Leslie AG, Walker JE, Nat Struct Biol. 2003 Sep;10(9):744-50. Epub 2003 Aug 17. PMID:12923572
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