2zbd

File:2zbd.jpg

spinqualitylabelsall models 2zbd, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of the SR Calcium Pump with Bound Aluminium Fluoride, ADP and Calcium

OverviewOverview

P-type ion transporting ATPases are ATP-powered ion pumps that establish, ion concentration gradients across biological membranes. Transfer of bound, cations to the lumenal or extracellular side occurs while the ATPase is, phosphorylated. Here we report at 2.3 A resolution the structure of the, calcium-ATPase of skeletal muscle sarcoplasmic reticulum, a representative, P-type ATPase that is crystallized in the absence of Ca2+ but in the, presence of magnesium fluoride, a stable phosphate analogue. This and, other crystal structures determined previously provide atomic models for, all four principal states in the reaction cycle. These structures show, that the three cytoplasmic domains rearrange to move six out of ten, transmembrane helices, thereby changing the affinity of the Ca2+-binding, sites and the gating of the ion pathway. Release of ADP triggers the, opening of the lumenal gate and release of phosphate its closure, effected, mainly through movement of the A-domain, the actuator of transmembrane, gates.

About this StructureAbout this Structure

2ZBD is a Single protein structure of sequence from Oryctolagus cuniculus with CA, MG, ALF, ACE, ADP and PC1 as ligands. This structure superseeds the now removed PDB entries 2Z9R and 1WPE. Active as Calcium-transporting ATPase, with EC number 3.6.3.8 Full crystallographic information is available from OCA.

ReferenceReference

Lumenal gating mechanism revealed in calcium pump crystal structures with phosphate analogues., Toyoshima C, Nomura H, Tsuda T, Nature. 2004 Nov 18;432(7015):361-8. Epub 2004 Sep 26. PMID:15448704

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