1m6r

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Revision as of 04:45, 25 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="1m6r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1m6r, resolution 1.54Å" /> '''Crystal structure of...)
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File:1m6r.gif


1m6r, resolution 1.54Å

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Crystal structure of rGd(CGCGCG) forming hexamer Z-DNA duplex with 5'-(rG) overhang

OverviewOverview

The crystal structure of the heptamer rGd(CGCGCG) has been determined at, 1.54 A resolution with R(work) and R(free) of 0.191 and 0.235, respectively. The crystal belongs to space group P2(1)2(1)2(1), with, unit-cell parameters a = 17.96, b = 31.47, c = 44.73 A and two independent, strands in the asymmetric unit. The chimera forms a Z-DNA hexamer duplex, d(CGCGCG)(2), with the 5'-overhang rG invisible in the density. The, replacement of rG in the 5' terminus of d(GCGCGCG) changes the reverse, Hoogsteen G.G base pairing of the 5'-overhang dG (Pan et al., 1997)., However, the replacement does not change the helix from Z-form to A-form, or the water-structure motifs in the Z-DNA double-helical structure.

About this StructureAbout this Structure

1M6R is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of rGd(CGCGCG): a Z-DNA hexamer duplex with a 5'-(rG) overhang., Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2003 Mar;59(Pt 3):433-7. Epub 2003, Feb 21. PMID:12595699

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