1d49
|
THE STRUCTURE OF A B-DNA DECAMER WITH A CENTRAL T-A STEP: C-G-A-T-T-A-A-T-C-G
OverviewOverview
The X-ray crystal structure analysis of the decamer C-G-A-T-T-A-A-T-C-G, has been carried out to a resolution of 1.5 A. The crystals are space, group P2(1)2(1)2(1), cell dimensions a = 38.60 A, b = 39.10 A, c = 33.07, A. The structure was solved by molecular replacement and refined with, X-PLOR and NUCLSQ. The final R factor for a model with 404 DNA atoms, 108, water molecules and one magnesium hexahydrate cation is 15.7%. The double, helix is essentially isostructural with C-G-A-T-C-G-A-T-C-G, with closely, similar local helix parameters. The structure of the T-T-A-A center, differs from that found in C-G-C-G-T-T-A-A-C-G-C-G in that the minor, groove in our decamer is wide at the central T-A step rather than narrow, and the twist angle of the T-A step is small (31.1 degrees) rather than, large. Whereas the tetrad model provides a convenient framework for, discussing local DNA helix structure, it cannot be the entire story. The, articulated helix model of DNA structure proposes that certain sequence, regions of DNA show preferential twisting or bending properties, whereas, other regions are less capable of deformation, in a manner that may be, useful in sequence recognition by drugs and protein. Further crystal, structure analyses should help to delineate the precise nature of, sequence-dependent articulation in the DNA double helix.
About this StructureAbout this Structure
1D49 is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a B-DNA decamer with a central T-A step: C-G-A-T-T-A-A-T-C-G., Quintana JR, Grzeskowiak K, Yanagi K, Dickerson RE, J Mol Biol. 1992 May 20;225(2):379-95. PMID:1593626
Page seeded by OCA on Sun Nov 25 02:28:24 2007