1ecp
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PURINE NUCLEOSIDE PHOSPHORYLASE
OverviewOverview
BACKGROUND: Purine nucleoside phosphorylase (PNP) from Escherichia coli is, a hexameric enzyme that catalyzes the reversible phosphorolysis of 6-amino, and 6-oxopurine (2'-deoxy)ribonucleosides to the free base and, (2'-deoxy)ribose-1-phosphate. In contrast, human and bovine PNPs are, trimeric and accept only 6-oxopurine nucleosides as substrates. The, difference in the specificities of these two enzymes has been utilized in, gene therapy treatments in which certain prodrugs are cleaved by E. coli, PNP but not the human enzyme. The trimeric and hexameric PNPs show no, similarity in amino acid sequence, even though they catalyze the same, basic chemical reaction. Structural comparison of the active sites of, mammalian and E. coli PNPs would provide an improved basis for the design, of ... [(full description)]
About this StructureAbout this Structure
1ECP is a [Single protein] structure of sequence from [Escherichia coli]. Active as [Transferase], with EC number [2.4.2.1]. Structure known Active Sites: S1 and S2. Full crystallographic information is available from [OCA].
ReferenceReference
The crystal structure of Escherichia coli purine nucleoside phosphorylase: a comparison with the human enzyme reveals a conserved topology., Mao C, Cook WJ, Zhou M, Koszalka GW, Krenitsky TA, Ealick SE, Structure. 1997 Oct 15;5(10):1373-83. PMID:9351810
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