1tan
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TANDEM DNA, NMR, MINIMIZED AVERAGE STRUCTURE
OverviewOverview
The solution structure of an estrone (Es)-tethered tandem DNA duplex, consisting of two Es-tethered tetranucleotides and a target octameric DNA, sequence is reported. The structure of this Es-tethered tandem duplex has, been compared with a corresponding natural tandem duplex without estrones., The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex, increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered, tetranucleotide part increases by 7 degrees C, compared with the, corresponding natural counterpart. The NMR structures of both the, Es-tethered tandem duplex and the natural counterpart have been based on, 24 experimental NMR constraints per residue. Despite the fact that there, is considerable distortion at the junction of two Es-tethered, tetranucleotides in the major groove of the Es-tethered DNA duplex, compared to the natural counterpart, both duplexes do take up B-type DNA, structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be, responsible for the increase of the thermal stability of the Es-tethered, tandem duplex in comparison with the natural counterpart.
About this StructureAbout this Structure
1TAN is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
ReferenceReference
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3')., Denisov AY, Sandstrom A, Maltseva TV, Pyshnyi DV, Ivanova EM, Zarytova VF, Chattopadhyaya J, J Biomol Struct Dyn. 1997 Dec;15(3):499-516. PMID:9439997
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