8te8

Crystal Structure of Pyridoxal Reductase (PDXI)Crystal Structure of Pyridoxal Reductase (PDXI)

Structural highlights

8te8 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PDXI_ECOLI Catalyzes the NAD(P)H-dependent reduction of pyridoxal to pyridoxine in vitro. Is not able to reduce 4-pyridoxate, and to oxidize pyridoxine or pyridoxamine (PubMed:27941785). Has Kemp eliminase activity towards the non-physiological substrate 5-nitrobenzisoxazole, producing 4-nitro-2-cyanophenol; this activity is not considered to be physiologically relevant (PubMed:21332126).^[1] ^[2]

References

↑ Khersonsky O, Malitsky S, Rogachev I, Tawfik DS. Role of chemistry versus substrate binding in recruiting promiscuous enzyme functions. Biochemistry. 2011 Apr 5;50(13):2683-90. PMID:21332126 doi:10.1021/bi101763c
↑ Sevin DC, Fuhrer T, Zamboni N, Sauer U. Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli. Nat Methods. 2017 Feb;14(2):187-194. doi: 10.1038/nmeth.4103. Epub 2016 Dec 12. PMID:27941785 doi:http://dx.doi.org/10.1038/nmeth.4103

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OCA

[1] Khersonsky O, Malitsky S, Rogachev I, Tawfik DS. Role of chemistry versus substrate binding in recruiting promiscuous enzyme functions. Biochemistry. 2011 Apr 5;50(13):2683-90. PMID:21332126 doi:10.1021/bi101763c

[2] Sevin DC, Fuhrer T, Zamboni N, Sauer U. Nontargeted in vitro metabolomics for high-throughput identification of novel enzymes in Escherichia coli. Nat Methods. 2017 Feb;14(2):187-194. doi: 10.1038/nmeth.4103. Epub 2016 Dec 12. PMID:27941785 doi:http://dx.doi.org/10.1038/nmeth.4103

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