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Structure of NOT1:NOT10:NOT11 module of the chicken CCR4-NOT complexStructure of NOT1:NOT10:NOT11 module of the chicken CCR4-NOT complex
Structural highlights
FunctionCNO10_CHICK Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex (By similarity). Publication Abstract from PubMedNOT1, NOT10, and NOT11 form a conserved module in the CCR4-NOT complex, critical for post-transcriptional regulation in eukaryotes, but how this module contributes to the functions of the CCR4-NOT remains poorly understood. Here, we present cryo-EM structures of human and chicken NOT1:NOT10:NOT11 ternary complexes to sub-3 A resolution, revealing an evolutionarily conserved, flexible structure. Through biochemical dissection studies, which include the Drosophila orthologs, we show that the module assembly is hierarchical, with NOT11 binding to NOT10, which then organizes it for binding to NOT1. A short proline-rich motif in NOT11 stabilizes the entire module assembly. Structure and assembly of the NOT10:11 module of the CCR4-NOT complex.,Levdansky Y, Raisch T, Deme JC, Pekovic F, Elmlund H, Lea SM, Valkov E Commun Biol. 2023 Jul 17;6(1):739. doi: 10.1038/s42003-023-05122-4. PMID:37460791[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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