7jlu

Structure of the activated Roq1 resistosome directly recognizing the pathogen effector XopQStructure of the activated Roq1 resistosome directly recognizing the pathogen effector XopQ

Structural highlights

7jlu is a 2 chain structure with sequence from Nicotiana benthamiana and Xanthomonas euvesicatoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ROQ1_NICBE Disease resistance (R) protein that specifically recognizes the Xanthomonas and Pseudomonas effector proteins XopQ and HopQ1, and triggers cell death (PubMed:28891100). Acts as a NAD(+) hydrolase (NADase): in response to activation, catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR) and nicotinamide; NAD(+) cleavage triggering a defense system that promotes cell death (PubMed:31439792).^[1] ^[2]

References

↑ Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B. Roq1 mediates recognition of the Xanthomonas and Pseudomonas effector proteins XopQ and HopQ1. Plant J. 2017 Dec;92(5):787-795. doi: 10.1111/tpj.13715. Epub 2017 Oct 25. PMID:28891100 doi:http://dx.doi.org/10.1111/tpj.13715
↑ Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B. NAD(+) cleavage activity by animal and plant TIR domains in cell death pathways. Science. 2019 Aug 23;365(6455):793-799. doi: 10.1126/science.aax1911. PMID:31439792 doi:http://dx.doi.org/10.1126/science.aax1911

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OCA

[1] Schultink A, Qi T, Lee A, Steinbrenner AD, Staskawicz B. Roq1 mediates recognition of the Xanthomonas and Pseudomonas effector proteins XopQ and HopQ1. Plant J. 2017 Dec;92(5):787-795. doi: 10.1111/tpj.13715. Epub 2017 Oct 25. PMID:28891100 doi:http://dx.doi.org/10.1111/tpj.13715

[2] Horsefield S, Burdett H, Zhang X, Manik MK, Shi Y, Chen J, Qi T, Gilley J, Lai JS, Rank MX, Casey LW, Gu W, Ericsson DJ, Foley G, Hughes RO, Bosanac T, von Itzstein M, Rathjen JP, Nanson JD, Boden M, Dry IB, Williams SJ, Staskawicz BJ, Coleman MP, Ve T, Dodds PN, Kobe B. NAD(+) cleavage activity by animal and plant TIR domains in cell death pathways. Science. 2019 Aug 23;365(6455):793-799. doi: 10.1126/science.aax1911. PMID:31439792 doi:http://dx.doi.org/10.1126/science.aax1911

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