7cvq

crystal structure of Arabidopsis CO CCT domain in complex with NF-YB2/YC3 and FT CORE1 DNAcrystal structure of Arabidopsis CO CCT domain in complex with NF-YB2/YC3 and FT CORE1 DNA

Structural highlights

7cvq is a 16 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CONS_ARATH Transcription factor that acts in the long day flowering pathway and may mediate between the circadian clock and the control of flowering. Plays a role in the regulation of flowering time by acting on 'SUPPRESSOR OF OVEREXPRESSION OF CO1', 'TERMINAL FLOWER 1' and 'FLOWERING LOCUS T'. Also regulates P5CS2 and ACS10 (involved in proline and ethylene biosynthesis, respectively). Regulates the expression of NAKR1 by binding to the 5'-TGTG(N2-3)ATG-3' motif (PubMed:27255839).^[1] ^[2] ^[3] ^[4] NFYC3_ARATH Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters.

Publication Abstract from PubMed

Flowering plants sense various environmental and endogenous signals to trigger the floral transition and start the reproductive growth cycle. CONSTANS (CO) is a master transcription factor in the photoperiod floral pathway that integrates upstream signals and activates the florigen gene FLOWERING LOCUS T (FT). Here, we performed comprehensive structural and biochemical analyses to study the molecular mechanism underlying the regulation of FT by CO in Arabidopsis thaliana. We show that the four previously characterized cis-elements in the FT promoter proximal region, CORE1, CORE2, P1, and P2, are all direct CO binding sites. Structural analysis of CO in complex with NUCLEAR FACTOR-YB/YC (NF-YB/YC) and the CORE2 or CORE1 elements revealed the molecular basis for the specific recognition of the shared TGTG motifs. Biochemical analysis suggested that CO might form a homomultimeric assembly via its N-terminal B-Box domain and simultaneously occupy multiple cis-elements within the FT promoter. We suggest that this multivalent binding gives the CO-NF-Y complex high affinity and specificity for FT promoter binding. Overall, our data provide a detailed molecular model for the regulation of FT by the master transcription factor complex CO-NF-Y during the floral transition.

Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex.,Lv X, Zeng X, Hu H, Chen L, Zhang F, Liu R, Liu Y, Zhou X, Wang C, Wu Z, Kim C, He Y, Du J Plant Cell. 2021 May 31;33(4):1182-1195. doi: 10.1093/plcell/koab016. PMID:33693873^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF, Coupland G. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science. 2000 Jun 2;288(5471):1613-6. PMID:10834834
↑ Suarez-Lopez P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature. 2001 Apr 26;410(6832):1116-20. doi: 10.1038/35074138. PMID:11323677 doi:http://dx.doi.org/10.1038/35074138
↑ Song YH, Lee I, Lee SY, Imaizumi T, Hong JC. CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis. Plant J. 2012 Jan;69(2):332-42. doi: 10.1111/j.1365-313X.2011.04793.x. Epub 2011 , Nov 18. PMID:21950734 doi:http://dx.doi.org/10.1111/j.1365-313X.2011.04793.x
↑ Zhu Y, Liu L, Shen L, Yu H. NaKR1 regulates long-distance movement of FLOWERING LOCUS T in Arabidopsis. Nat Plants. 2016 May 27;2(6):16075. doi: 10.1038/nplants.2016.75. PMID:27255839 doi:http://dx.doi.org/10.1038/nplants.2016.75
↑ Lv X, Zeng X, Hu H, Chen L, Zhang F, Liu R, Liu Y, Zhou X, Wang C, Wu Z, Kim C, He Y, Du J. Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex. Plant Cell. 2021 May 31;33(4):1182-1195. PMID:33693873 doi:10.1093/plcell/koab016

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OCA

[1] Samach A, Onouchi H, Gold SE, Ditta GS, Schwarz-Sommer Z, Yanofsky MF, Coupland G. Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Science. 2000 Jun 2;288(5471):1613-6. PMID:10834834

[2] Suarez-Lopez P, Wheatley K, Robson F, Onouchi H, Valverde F, Coupland G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis. Nature. 2001 Apr 26;410(6832):1116-20. doi: 10.1038/35074138. PMID:11323677 doi:http://dx.doi.org/10.1038/35074138

[3] Song YH, Lee I, Lee SY, Imaizumi T, Hong JC. CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis. Plant J. 2012 Jan;69(2):332-42. doi: 10.1111/j.1365-313X.2011.04793.x. Epub 2011 , Nov 18. PMID:21950734 doi:http://dx.doi.org/10.1111/j.1365-313X.2011.04793.x

[4] Zhu Y, Liu L, Shen L, Yu H. NaKR1 regulates long-distance movement of FLOWERING LOCUS T in Arabidopsis. Nat Plants. 2016 May 27;2(6):16075. doi: 10.1038/nplants.2016.75. PMID:27255839 doi:http://dx.doi.org/10.1038/nplants.2016.75

[5] Lv X, Zeng X, Hu H, Chen L, Zhang F, Liu R, Liu Y, Zhou X, Wang C, Wu Z, Kim C, He Y, Du J. Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex. Plant Cell. 2021 May 31;33(4):1182-1195. PMID:33693873 doi:10.1093/plcell/koab016

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