6cbj

Crystal Structure of DH270.3 Fab in complex with Man9Crystal Structure of DH270.3 Fab in complex with Man9

Structural highlights

6cbj is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.85Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

S6C4S0_HUMAN

Publication Abstract from PubMed

HIV-1 envelope (Env) mimetics are candidate components of prophylactic vaccines and potential therapeutics. Here we use a synthetic V3-glycopeptide ("Man9-V3") for structural studies of an HIV Env third variable loop (V3)-glycan directed, broadly neutralizing antibody (bnAb) lineage ("DH270"), to visualize the epitope on Env and to study how affinity maturation of the lineage proceeded. Unlike many previous V3 mimetics, Man9-V3 encompasses two key features of the V3 region recognized by V3-glycan bnAbs-the conserved GDIR motif and the N332 glycan. In our structure of an antibody fragment of a lineage member, DH270.6, in complex with the V3 glycopeptide, the conformation of the antibody-bound glycopeptide conforms closely to that of the corresponding segment in an intact HIV-1 Env trimer. An additional structure identifies roles for two critical mutations in the development of breadth. The results suggest a strategy for use of a V3 glycopeptide as a vaccine immunogen.

HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope.,Fera D, Lee MS, Wiehe K, Meyerhoff RR, Piai A, Bonsignori M, Aussedat B, Walkowicz WE, Ton T, Zhou JO, Danishefsky S, Haynes BF, Harrison SC Nat Commun. 2018 Mar 16;9(1):1111. doi: 10.1038/s41467-018-03565-6. PMID:29549260^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fera D, Lee MS, Wiehe K, Meyerhoff RR, Piai A, Bonsignori M, Aussedat B, Walkowicz WE, Ton T, Zhou JO, Danishefsky S, Haynes BF, Harrison SC. HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope. Nat Commun. 2018 Mar 16;9(1):1111. doi: 10.1038/s41467-018-03565-6. PMID:29549260 doi:http://dx.doi.org/10.1038/s41467-018-03565-6

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OCA

[1] Fera D, Lee MS, Wiehe K, Meyerhoff RR, Piai A, Bonsignori M, Aussedat B, Walkowicz WE, Ton T, Zhou JO, Danishefsky S, Haynes BF, Harrison SC. HIV envelope V3 region mimic embodies key features of a broadly neutralizing antibody lineage epitope. Nat Commun. 2018 Mar 16;9(1):1111. doi: 10.1038/s41467-018-03565-6. PMID:29549260 doi:http://dx.doi.org/10.1038/s41467-018-03565-6

[1]