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Structure-Guided Engineering of Reductase: Efficient Attenuating Substrate Inhibition in Asymmetric CatalysisStructure-Guided Engineering of Reductase: Efficient Attenuating Substrate Inhibition in Asymmetric Catalysis
Structural highlights
FunctionPublication Abstract from PubMedSubstrate inhibition of enzymes is one of the main obstacles encountered frequently in industrial biocatalysis. Haloketone reductase SsCR was seriously inhibited by substrate 2,2',4'-trichloroacetophenone. In this study, two essential loops were found that have a relationship with substrate binding by conducting X-ray crystal structure analysis. Three key residues were selected from the tips of the loops and substituted with amino acids with lower hydrophobicity to weaken the hydrophobic interactions that bridge the two loops, resulting in a remarkable reduction of substrate inhibition. Among these variants, L211H showed a significant attenuation of substrate inhibition, with a Ki of 16 mM, which was 16 times that of the native enzyme. The kinetic parameter kcat/Km of L211H was 3.1 x 10(3) s(-1) mM(-1), showing the comparable catalytic efficiency to that of the wild-type enzyme (WT). At the substrate loading of 100 mM, the space time yield of variant L211H in asymmetric reduction of the haloketone was 3-fold higher than that of the WT. Attenuated substrate inhibition of a haloketone reductase via structure-guided loop engineering.,Shang YP, Chen Q, Li AT, Quan S, Xu JH, Yu HL J Biotechnol. 2020 Jan 20;308:141-147. doi: 10.1016/j.jbiotec.2019.12.011. Epub, 2019 Dec 19. PMID:31866427[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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