5yks

Crystal structure of sucrose nonfermenting-related kinase (SNRK)Crystal structure of sucrose nonfermenting-related kinase (SNRK)

Structural highlights

5yks is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SNRK_HUMAN May play a role in hematopoietic cell proliferation or differentiation. Potential mediator of neuronal apoptosis.[UniProtKB:Q63553]^[1] ^[2]

Publication Abstract from PubMed

Sucrose non-fermenting (Snf1)-related kinase (SNRK) is a novel member of the AMP-activated protein kinase (AMPK) family and is involved in many metabolic processes. Here we report the crystal structure of an N-terminal SNRK fragment containing kinase and adjacent ubiquitin-associated (UBA) domains. This structure shows that the UBA domain binds between the N- and C-lobes of the kinase domain. The mode of UBA binding in SNRK largely resembles that in AMPK and brain specific kinase (BRSK), however, unique interactions play vital roles in stabilizing the KD-UBA interface of SNRK. We further propose a potential role of the UBA domain in the regulation of SNRK kinase activity. This study provides new insights into the structural diversities of the AMPK kinase family.

Crystal structure of the kinase and UBA domains of SNRK reveals a distinct UBA binding mode in the AMPK family.,Wang YL, Wang J, Chen X, Wang ZX, Wu JW Biochem Biophys Res Commun. 2017 Oct 20. pii: S0006-291X(17)32083-1. doi:, 10.1016/j.bbrc.2017.10.105. PMID:29061304^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kertesz N, Samson J, Debacker C, Wu H, Labastie MC. Cloning and characterization of human and mouse SNRK sucrose non-fermenting protein (SNF-1)-related kinases. Gene. 2002 Jul 10;294(1-2):13-24. PMID:12234663
↑ Jaleel M, McBride A, Lizcano JM, Deak M, Toth R, Morrice NA, Alessi DR. Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate. FEBS Lett. 2005 Feb 28;579(6):1417-23. PMID:15733851 doi:S0014-5793(05)00141-9
↑ Wang YL, Wang J, Chen X, Wang ZX, Wu JW. Crystal structure of the kinase and UBA domains of SNRK reveals a distinct UBA binding mode in the AMPK family. Biochem Biophys Res Commun. 2017 Oct 20. pii: S0006-291X(17)32083-1. doi:, 10.1016/j.bbrc.2017.10.105. PMID:29061304 doi:http://dx.doi.org/10.1016/j.bbrc.2017.10.105

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OCA

[1] Kertesz N, Samson J, Debacker C, Wu H, Labastie MC. Cloning and characterization of human and mouse SNRK sucrose non-fermenting protein (SNF-1)-related kinases. Gene. 2002 Jul 10;294(1-2):13-24. PMID:12234663

[2] Jaleel M, McBride A, Lizcano JM, Deak M, Toth R, Morrice NA, Alessi DR. Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate. FEBS Lett. 2005 Feb 28;579(6):1417-23. PMID:15733851 doi:S0014-5793(05)00141-9

[3] Wang YL, Wang J, Chen X, Wang ZX, Wu JW. Crystal structure of the kinase and UBA domains of SNRK reveals a distinct UBA binding mode in the AMPK family. Biochem Biophys Res Commun. 2017 Oct 20. pii: S0006-291X(17)32083-1. doi:, 10.1016/j.bbrc.2017.10.105. PMID:29061304 doi:http://dx.doi.org/10.1016/j.bbrc.2017.10.105

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