5a3e

2.5A structure of lysozyme determined by MicroED with data from a single crystal2.5A structure of lysozyme determined by MicroED with data from a single crystal

Structural highlights

5a3e is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron crystallography, Resolution 2.501Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.^[1]

Publication Abstract from PubMed

MicroED uses very small three-dimensional protein crystals and electron diffraction for structure determination. We present an improved data collection protocol for MicroED called 'continuous rotation'. Microcrystals are continuously rotated during data collection, yielding more accurate data. The method enables data processing with the crystallographic software tool MOSFLM, which resulted in improved resolution for the model protein lysozyme. These improvements are paving the way for the broad implementation and application of MicroED in structural biology.

High-resolution structure determination by continuous-rotation data collection in MicroED.,Nannenga BL, Shi D, Leslie AG, Gonen T Nat Methods. 2014 Aug 3. doi: 10.1038/nmeth.3043. PMID:25086503^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
↑ Nannenga BL, Shi D, Leslie AG, Gonen T. High-resolution structure determination by continuous-rotation data collection in MicroED. Nat Methods. 2014 Aug 3. doi: 10.1038/nmeth.3043. PMID:25086503 doi:http://dx.doi.org/10.1038/nmeth.3043

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OCA

[1] Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021

[2] Nannenga BL, Shi D, Leslie AG, Gonen T. High-resolution structure determination by continuous-rotation data collection in MicroED. Nat Methods. 2014 Aug 3. doi: 10.1038/nmeth.3043. PMID:25086503 doi:http://dx.doi.org/10.1038/nmeth.3043

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