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Crystal structure of Chaetomium thermophilum MnSODCrystal structure of Chaetomium thermophilum MnSOD
Structural highlights
FunctionF8V325_9PEZI Destroys radicals which are normally produced within the cells and which are toxic to biological systems.[RuleBase:RU000414] Publication Abstract from PubMedA manganese superoxide dismutase from the thermophilic fungus Chaetomium thermophilum (CtMnSOD) was expressed in Pichia pastoris and purified to homogeneity. Its optimal temperature was 60 degrees C with approximately 75% of its activity retained after incubation at 70 degrees C for 60min. Recombinant yeast cells carrying C. thermophilum mnsod gene exhibited higher stress resistance to salt and oxidative stress-inducing agents than control yeast cells. In an effort to provide structural insights, CtMnSOD was crystallized and its structure was determined at 2.0A resolution. The overall architecture of CtMnSOD was found similar to other MnSODs with highest structural similarities obtained against a MnSOD from the thermophilic fungus Aspergillus fumigatus. In order to explain its thermostability, structural and sequence analysis of CtMnSOD with other MnSODs was carried out. An increased number of charged residues and an increase in the number of intersubunit salt bridges and the Thr:Ser ratio were identified as potential reasons for the thermostability of CtMnSOD. Crystal structure and biochemical characterization of a manganese superoxide dismutase from Chaetomium thermophilum.,Haikarainen T, Frioux C, Zhnag LQ, Li DC, Papageorgiou AC Biochim Biophys Acta. 2013 Dec 3. pii: S1570-9639(13)00420-2. doi:, 10.1016/j.bbapap.2013.11.014. PMID:24316252[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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