3twm

Crystal structure of Arabidopsis thaliana FPGCrystal structure of Arabidopsis thaliana FPG

Structural highlights

3twm is a 6 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.8Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FPG_ARATH Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Can process efficiently 4,6-diamino-5-formamidopyrimidine (FapyA), 2,6-diamino-4- hydroxy-5-formamidopyrimidine (FapyG) and the further oxidation products of 8-oxoguanine (8-oxoG), such as guanidinohydantoin and spiroiminodihydantoin. Has marginal activity towards 8-oxoG. Has AP (apurinic/apyrimidinic) lyase activity. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.^[1] ^[2] ^[3]

References

↑ Gao MJ, Murphy TM. Alternative forms of formamidopyrimidine-DNA glycosylase from Arabidopsis thaliana. Photochem Photobiol. 2001 Feb;73(2):128-34. PMID:11272725
↑ Duclos S, Aller P, Jaruga P, Dizdaroglu M, Wallace SS, Doublie S. Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine. DNA Repair (Amst). 2012 Jul 10. PMID:22789755 doi:10.1016/j.dnarep.2012.06.004
↑ Ohtsubo T, Matsuda O, Iba K, Terashima I, Sekiguchi M, Nakabeppu Y. Molecular cloning of AtMMH, an Arabidopsis thaliana ortholog of the Escherichia coli mutM gene, and analysis of functional domains of its product. Mol Gen Genet. 1998 Oct;259(6):577-90. PMID:9819050

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OCA

[1] Gao MJ, Murphy TM. Alternative forms of formamidopyrimidine-DNA glycosylase from Arabidopsis thaliana. Photochem Photobiol. 2001 Feb;73(2):128-34. PMID:11272725

[2] Duclos S, Aller P, Jaruga P, Dizdaroglu M, Wallace SS, Doublie S. Structural and biochemical studies of a plant formamidopyrimidine-DNA glycosylase reveal why eukaryotic Fpg glycosylases do not excise 8-oxoguanine. DNA Repair (Amst). 2012 Jul 10. PMID:22789755 doi:10.1016/j.dnarep.2012.06.004

[3] Ohtsubo T, Matsuda O, Iba K, Terashima I, Sekiguchi M, Nakabeppu Y. Molecular cloning of AtMMH, an Arabidopsis thaliana ortholog of the Escherichia coli mutM gene, and analysis of functional domains of its product. Mol Gen Genet. 1998 Oct;259(6):577-90. PMID:9819050

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