Structure of an N-terminal truncation of Deinococcus radiodurans RecD2Structure of an N-terminal truncation of Deinococcus radiodurans RecD2
Structural highlights
3e1s is a 1 chain structure with sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
RECDL_DEIRA DNA-dependent ATPase (ssDNA better than dsDNA) and ATP-dependent 5'-3' DNA helicase. Appears to move along DNA in single base steps, powered by hydrolysis of 1 molecule of ATP. Has low processivity; short (20 bp) substrates with 5'-overhangs or forked ends are the best substrates, is much less efficient on 52 or 76 bp substrates with 5'- overhangs. The presence of single-stranded DNA-binding protein (SSB) increases unwinding 4-5 fold. Has no activity on blunt DNA or DNA with 3'-overhangs. Requires at least 10 bases of 5'-ssDNA for helicase activity.[1][2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑Wang J, Julin DA. DNA helicase activity of the RecD protein from Deinococcus radiodurans. J Biol Chem. 2004 Dec 10;279(50):52024-32. Epub 2004 Oct 4. PMID:15466873 doi:http://dx.doi.org/10.1074/jbc.M408645200
↑Saikrishnan K, Powell B, Cook NJ, Webb MR, Wigley DB. Mechanistic basis of 5'-3' translocation in SF1B helicases. Cell. 2009 May 29;137(5):849-59. PMID:19490894 doi:10.1016/j.cell.2009.03.036
