2yhe

Structure determination of the stereoselective inverting sec- alkylsulfatase Pisa1 from Pseudomonas sp.Structure determination of the stereoselective inverting sec- alkylsulfatase Pisa1 from Pseudomonas sp.

Structural highlights

2yhe is a 6 chain structure with sequence from Pseudomonas sp. DSM 6611. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RSAKS_PSESP Alkylsulfatase that catalyzes the enantioselective hydrolysis of secondary-alkylsulfates with strict inversion of configuration, leading to the formation of homochiral (S)-configurated alcohols and nonreacted sulfate esters (PubMed:21770430, Ref.2, PubMed:23061549). The substrate spectrum includes a range of linear, branched or cyclic sec-alkylsulfates (PubMed:21770430). Can use sec-alkylsulfate esters bearing aromatic, olefinic and acetylenic moieties (Ref.2). Acts by cleaving the C-O bond, resulting in inversion at the carbon (PubMed:23061549).^[1] ^[2] ^[3]

References

↑ Schober M, Gadler P, Knaus T, Kayer H, Birner-Grünberger R, Gülly C, Macheroux P, Wagner U, Faber K. A stereoselective inverting sec-alkylsulfatase for the deracemization of sec-alcohols. Org Lett. 2011 Aug 19;13(16):4296-9. PMID:21770430 doi:10.1021/ol201635y
↑ Knaus T, Schober M, Kepplinger B, Faccinelli M, Pitzer J, Faber K, Macheroux P, Wagner U. Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. FEBS J. 2012 Dec;279(23):4374-84. doi: 10.1111/febs.12027. Epub 2012 Nov 7. PMID:23061549 doi:http://dx.doi.org/10.1111/febs.12027
↑ Schober M, Gadler P, Knaus T, Kayer H, Birner-Grünberger R, Gülly C, Macheroux P, Wagner U, Faber K. A stereoselective inverting sec-alkylsulfatase for the deracemization of sec-alcohols. Org Lett. 2011 Aug 19;13(16):4296-9. PMID:21770430 doi:10.1021/ol201635y

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OCA

[1] Schober M, Gadler P, Knaus T, Kayer H, Birner-Grünberger R, Gülly C, Macheroux P, Wagner U, Faber K. A stereoselective inverting sec-alkylsulfatase for the deracemization of sec-alcohols. Org Lett. 2011 Aug 19;13(16):4296-9. PMID:21770430 doi:10.1021/ol201635y

[2] Knaus T, Schober M, Kepplinger B, Faccinelli M, Pitzer J, Faber K, Macheroux P, Wagner U. Structure and mechanism of an inverting alkylsulfatase from Pseudomonas sp. DSM6611 specific for secondary alkyl sulfates. FEBS J. 2012 Dec;279(23):4374-84. doi: 10.1111/febs.12027. Epub 2012 Nov 7. PMID:23061549 doi:http://dx.doi.org/10.1111/febs.12027

[3] Schober M, Gadler P, Knaus T, Kayer H, Birner-Grünberger R, Gülly C, Macheroux P, Wagner U, Faber K. A stereoselective inverting sec-alkylsulfatase for the deracemization of sec-alcohols. Org Lett. 2011 Aug 19;13(16):4296-9. PMID:21770430 doi:10.1021/ol201635y

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