Crystal structure of the short-chain dehydrogenase Galactitol- Dehydrogenase (GatDH) of Rhodobacter sphaeroides in complex with NADCrystal structure of the short-chain dehydrogenase Galactitol- Dehydrogenase (GatDH) of Rhodobacter sphaeroides in complex with NAD
Structural highlights
2wsb is a 4 chain structure with sequence from Cereibacter sphaeroides. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
GATDH_CERSP Catalyzes the interconversion of galactitol to the rare sugar L-tagatose (PubMed:7551050). Shows activity with a wide range of substrates, and catalyzes the oxidation of a variety of polyvalent aliphatic alcohols and polyols to the corresponding ketones and ketoses, respectively, and in the reverse reaction, it reduces ketones with high stereoselectivity yielding the corresponding S-configurated alcohols (PubMed:7551050, PubMed:15296184). Shows high activity with D-threitol, xylitol, 1,2-hexanediol, 1,2-pentanediol, 2-hexanol, L-erythrulose, D-ribulose and acetoin (PubMed:7551050). Specific for NAD(+) (PubMed:7551050).[1][2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑Kohring GW, Wiehr P, Jeworski M, Giffhorn F. Stereoselective oxidation of aliphatic diols and reduction of hydroxy-ketones with galactitol dehydrogenase from Rhodobacter sphaeroides D. Commun Agric Appl Biol Sci. 2003;68(2 Pt A):309-12 PMID:15296184
↑Schneider KH, Jakel G, Hoffmann R, Giffhorn F. Enzyme evolution in Rhodobacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme. Microbiology (Reading). 1995 Aug;141 ( Pt 8):1865-1873. PMID:7551050 doi:10.1099/13500872-141-8-1865
